Tetracysteine as a reporter for gene therapy / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 496-501, 2009.
Article
in En
| WPRIM
| ID: wpr-360636
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the feasibility of using tetracysteine (TC) reporter in gene therapy.</p><p><b>METHODS</b>Effects of TC reporter and conventional reporter genes encoding green fluorescence protein (GFP) and luciferase (Luc) on expression and function of the therapeutic gene MGMT(P140K) were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry (FCM).</p><p><b>RESULTS</b>The TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection.</p><p><b>CONCLUSION</b>TC is a new kind of reporter gene for lentiviral vector in future gene therapy.</p>
Full text:
1
Database:
WPRIM
Main subject:
Lymphocytes
/
Genetic Therapy
/
Gene Expression Regulation
/
Cricetulus
/
CHO Cells
/
Genes, Reporter
/
Lentivirus
/
Cysteine
/
Genetics
/
Metabolism
Limits:
Animals
/
Humans
Language:
En
Journal:
Biomedical and Environmental Sciences
Year:
2009
Document type:
Article